|
KMID : 1101420190510020214
|
|
Korean Journal of Clinical Laboratory Science
2019 Volume.51 No. 2 p.214 ~ p.220
|
|
|
Anti-bacterial Activity and Standardization on Hwangryunhaedok?tang
|
|
Lee Dae-Yeon
Yi Young-Woo
Kim Wan-Su
Sim Sun-Hyung
Kwon Pil-Seung
Lee In-Hee
|
|
|
Abstract
|
|
|
|
Hwangryunhaedok-tang (HT) is prescribed in oriental medicine to treat inflammation-related diseases. This study examined the anti-bacterial effect to select the index components of Traditional Chinese Medicine (TCM) HT. The disc diffusion method and minimum inhibition concentrations (MIC) tests were conducted to measure the antibacterial activity of HT. The strains were Staphylococcus aureus ATCC 25923 and Escherichia coli 23724. Berberine and geniposide were selected as the index components of HT and were analyzed quantitatively by high-performance liquid chromatography (HPLC). As a result, the inhibition ring was formed at 11.7±1.3 mm and 8.7±0.7 mm at HT 20 mg and 10 mg on S.aureusATCC 25923, respectively; and 8.0±0.0 mm at HT 20 mg on E.coli ATCC 23724. The MIC were 31.25 mg/mL in S.aureus ATCC 25923 and 125.0 mg/mL in E.coli ATCC 23724. In quantitative analysis for standardization, HT contained 16.55 mg/g of berberine and 81.85 mg/g of geniposide. Overall, when antibiotics are developed using natural products, they may reduce the side effects and inhibit resistant bacteria. If research on HT continuously proceeds, positive developments are expected to be made in oriental medicine as well as in various other industries.
|
|
|
KEYWORD
|
|
|
Antibacterial activity, Disc diffusion, HPLC, HPLCHwangryunhaedok-tang, HPLCMinimum inhibition concentrations
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|